Recombinant:Article Title: Topoisomerase 3b facilitates piRNA biogenesis to promote transposon silencing and germ cell development.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies a-mouse FLAG-M2 Sigma-Aldrich Cat# F3165; RRID:AB_259529 Mouse Tdrd3 Cell Signaling Cat# 5942S; RRID:AB_2797626 anti-vasa Developmental Studies Hybridoma Bank Cat# anti-vasa; RRID:AB_760351 anti-Hts 1B1 Developmental Studies Hybridoma Bank Cat# 1B1; RRID:AB_528070 anti-Top3b Lee et al. N/A anti-Tdrd3 Lee et al. N/A anti-Aub Nishida et al. N/A anti-Armi Cook et al. N/A anti-Yb Murota et al. N/A Chemicals, peptides, and recombinant proteins Shields and Sang M3 Insect Medium Sigma-Aldrich Cat# S3652-1L Fly Extract Drosophila Genomics Resource Center DGRC Stock 1645670 GlutaMAX gibco 35050061 Antibiotic-Antimycotic gibco 15240062 Glutathione Sigma-Aldrich G6013-25G Penicillin-Streptomycin gibco 15140122 Insulin Sigma-Aldrich I9278-5ml Paraformaldehyde Santa Cruz Biotechnology CAS 30525-89-4 Glutaraldehyde Sigma-Aldrich G7776-10ML X-Gal Solution Thermo Scientific R0941 TRIzol Invitrogen 15596026 SuperScript double-stranded cDNA synthesis kit Invitrogen 11917010 End-Repair Mix Qiagen Y9140-LC-L MinElute Reaction Cleanup kit Qiagen 28204 TBE-Urea Gels, 15% Invitrogen EC6885BOX Sodium Acetate Invitrogen AM9740 EDTA Invitrogen AM9260G Buffer EB Qiagen 19086 NEBNext Small RNA Library Prep kit New England Biolabs E7300 TaqMa MicroRNA Reverse Transcription Kit applied biosystems 4366597 Random Hexamers Invitrogen N8080127 SYBRTM Green Universal Master Mix applied biosystems 4309155 Triton X-100 Sigma-Aldrich X100-5ML HEPES gibco 15630080 MgCl2 invitrogen AM9530G NaCl invitrogen AM9760G Sucrose Sigma-Aldrich S0389-500G cOmplete Protease Inhibitor Cocktail Roche 11697498001 RNaseOU Recombinant Ribonuclease Inhibitor invitrogen 10777019 Anti-FLAG M2 Magnetic Beads Sigma-Aldrich M8823-1ML Chloroform Sigma-Aldrich C2432-25ML Critical commercial assays Mass spectrometry Taplin Biological Mass Spectrometry Facility https://taplin.hms.harvard.edu/ (Continued on next page) Cell Reports 44, 115495, April 22, 2025 19 ..
Article Title: Protocol to evaluate a lineage marking system in the Drosophila testis.
Article Snippet: The primary antibodies listed (anti-Vasa, 1b1, 7G10) are specific antibodies obtained from the DSHB and are critical for accurately identifying germline and niche cell populations. .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat monoclonal anti-Vasa (1:20) Developmental Studies Hybridoma Bank DSHB Cat# anti-vasa; RRID: AB_760351 Mouse monoclonal anti-hu-li tai shao (1:20) Developmental Studies Hybridoma Bank DSHB Cat# 1b1; RRID: AB_528070 Mouse monoclonal anti-Fasciculin III (1:40) Developmental Studies Hybridoma Bank DSHB Cat# 7G10 anti-Fasciclin III; RRID: AB_528238 Cyanine 3 goat anti-mouse IgG + IgM (H + L) (1:400) Jackson ImmunoResearch Labs RRID: AB_2338684 Cyanine 5 goat anti-rat IgG (H + L) (1:400) Jackson ImmunoResearch Labs RRID: AB_2338263 Chemicals, peptides, and recombinant proteins Formaldehyde, 16% Electron Microscopy Sciences Cat#15710 TritonX-100 Sigma Cat#X100-500ML 103 phosphate-buffered saline (PBS) Fisher Scientific Cat#BP3991 Bovine Serum Albumin (BSA) Sigma Cat#A2153-500G Poly-L-lysine Sigma Cat#P5899-5MG Schneider’s Drosophila medium Sigma Cat#21-720-024 Fetal Bovine Serum (FBS) Sigma Cat#NC3365964 Penicillin-Streptomycin-Glutamine (1003) Gibco (Fisher Scientific) Cat#10-378-016 VECTASHIELD with DAPI Vector Lab Cat#H-1200-10 Deposited data VCell math model Bener et al. 1 VCell model: Username: Boris; Model name: Inaba_dedifferentiation_model_public; https://vcell.org/run-vcell-software Experimental models: Organisms/strains D. melanogaster: nos-FRT-mCherrystop-FRT-Gal4, UAS-GFP (Reporter-1) Inaba lab stock 17 N/A D. melanogaster: UAS(FRT.stop)mCD8GFP (Reporter-2) Bloomington Drosophila Stock Center RRID: BDSC_ 30032 D. melanogaster: pVas-Vasa mCherry Gift from Yukiko Yamashita FBtp0065762 D. melanogaster: Bam-FLPD5 Bener et al. 1 RRID: BDSC_604597 Software and algorithms ZEN Microscopy Software ZEISS RRID:SCR_013672 ImageJ Fiji Schneider et al. 18 RRID:SCR_003070 https://imagej.net/software/fiji/ Virtual Cell Resasco et al. 19 ; Slepchenko and Loew. .. 20 RRID:SCR_007421 https://vcell.org/ Other Zeiss LSM800 confocal microscope with an Airyscan module Zeiss RRID:SCR_015963 35mm glass bottom dishes Nunc (Thermo Fisher) Cat#150680 Dissection microscope Leica M80 Fiber optic microscope illuminator Schott KL 1600 Dumont #55 forceps Fine Science Tools 1125520 Pyrex spot plate with nine depressions Corning Cat#7220-85 Drosophila flypads Genesee Scientific Cat#59-119 Drosophila CO 2 bubbler kits Genesee Scientific Cat#59-181 4 STAR Protocols 7, 104479, June 19, 2026 MATERIALS AND EQUIPMENT (A) An illustration of the Bam-FLPD5 lineage tracing system.
cDNA Synthesis:Article Title: Topoisomerase 3b facilitates piRNA biogenesis to promote transposon silencing and germ cell development.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies a-mouse FLAG-M2 Sigma-Aldrich Cat# F3165; RRID:AB_259529 Mouse Tdrd3 Cell Signaling Cat# 5942S; RRID:AB_2797626 anti-vasa Developmental Studies Hybridoma Bank Cat# anti-vasa; RRID:AB_760351 anti-Hts 1B1 Developmental Studies Hybridoma Bank Cat# 1B1; RRID:AB_528070 anti-Top3b Lee et al. N/A anti-Tdrd3 Lee et al. N/A anti-Aub Nishida et al. N/A anti-Armi Cook et al. N/A anti-Yb Murota et al. N/A Chemicals, peptides, and recombinant proteins Shields and Sang M3 Insect Medium Sigma-Aldrich Cat# S3652-1L Fly Extract Drosophila Genomics Resource Center DGRC Stock 1645670 GlutaMAX gibco 35050061 Antibiotic-Antimycotic gibco 15240062 Glutathione Sigma-Aldrich G6013-25G Penicillin-Streptomycin gibco 15140122 Insulin Sigma-Aldrich I9278-5ml Paraformaldehyde Santa Cruz Biotechnology CAS 30525-89-4 Glutaraldehyde Sigma-Aldrich G7776-10ML X-Gal Solution Thermo Scientific R0941 TRIzol Invitrogen 15596026 SuperScript double-stranded cDNA synthesis kit Invitrogen 11917010 End-Repair Mix Qiagen Y9140-LC-L MinElute Reaction Cleanup kit Qiagen 28204 TBE-Urea Gels, 15% Invitrogen EC6885BOX Sodium Acetate Invitrogen AM9740 EDTA Invitrogen AM9260G Buffer EB Qiagen 19086 NEBNext Small RNA Library Prep kit New England Biolabs E7300 TaqMa MicroRNA Reverse Transcription Kit applied biosystems 4366597 Random Hexamers Invitrogen N8080127 SYBRTM Green Universal Master Mix applied biosystems 4309155 Triton X-100 Sigma-Aldrich X100-5ML HEPES gibco 15630080 MgCl2 invitrogen AM9530G NaCl invitrogen AM9760G Sucrose Sigma-Aldrich S0389-500G cOmplete Protease Inhibitor Cocktail Roche 11697498001 RNaseOU Recombinant Ribonuclease Inhibitor invitrogen 10777019 Anti-FLAG M2 Magnetic Beads Sigma-Aldrich M8823-1ML Chloroform Sigma-Aldrich C2432-25ML Critical commercial assays Mass spectrometry Taplin Biological Mass Spectrometry Facility https://taplin.hms.harvard.edu/ (Continued on next page) Cell Reports 44, 115495, April 22, 2025 19 ..
Reverse Transcription:Article Title: Topoisomerase 3b facilitates piRNA biogenesis to promote transposon silencing and germ cell development.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies a-mouse FLAG-M2 Sigma-Aldrich Cat# F3165; RRID:AB_259529 Mouse Tdrd3 Cell Signaling Cat# 5942S; RRID:AB_2797626 anti-vasa Developmental Studies Hybridoma Bank Cat# anti-vasa; RRID:AB_760351 anti-Hts 1B1 Developmental Studies Hybridoma Bank Cat# 1B1; RRID:AB_528070 anti-Top3b Lee et al. N/A anti-Tdrd3 Lee et al. N/A anti-Aub Nishida et al. N/A anti-Armi Cook et al. N/A anti-Yb Murota et al. N/A Chemicals, peptides, and recombinant proteins Shields and Sang M3 Insect Medium Sigma-Aldrich Cat# S3652-1L Fly Extract Drosophila Genomics Resource Center DGRC Stock 1645670 GlutaMAX gibco 35050061 Antibiotic-Antimycotic gibco 15240062 Glutathione Sigma-Aldrich G6013-25G Penicillin-Streptomycin gibco 15140122 Insulin Sigma-Aldrich I9278-5ml Paraformaldehyde Santa Cruz Biotechnology CAS 30525-89-4 Glutaraldehyde Sigma-Aldrich G7776-10ML X-Gal Solution Thermo Scientific R0941 TRIzol Invitrogen 15596026 SuperScript double-stranded cDNA synthesis kit Invitrogen 11917010 End-Repair Mix Qiagen Y9140-LC-L MinElute Reaction Cleanup kit Qiagen 28204 TBE-Urea Gels, 15% Invitrogen EC6885BOX Sodium Acetate Invitrogen AM9740 EDTA Invitrogen AM9260G Buffer EB Qiagen 19086 NEBNext Small RNA Library Prep kit New England Biolabs E7300 TaqMa MicroRNA Reverse Transcription Kit applied biosystems 4366597 Random Hexamers Invitrogen N8080127 SYBRTM Green Universal Master Mix applied biosystems 4309155 Triton X-100 Sigma-Aldrich X100-5ML HEPES gibco 15630080 MgCl2 invitrogen AM9530G NaCl invitrogen AM9760G Sucrose Sigma-Aldrich S0389-500G cOmplete Protease Inhibitor Cocktail Roche 11697498001 RNaseOU Recombinant Ribonuclease Inhibitor invitrogen 10777019 Anti-FLAG M2 Magnetic Beads Sigma-Aldrich M8823-1ML Chloroform Sigma-Aldrich C2432-25ML Critical commercial assays Mass spectrometry Taplin Biological Mass Spectrometry Facility https://taplin.hms.harvard.edu/ (Continued on next page) Cell Reports 44, 115495, April 22, 2025 19 ..
Protease Inhibitor:Article Title: Topoisomerase 3b facilitates piRNA biogenesis to promote transposon silencing and germ cell development.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies a-mouse FLAG-M2 Sigma-Aldrich Cat# F3165; RRID:AB_259529 Mouse Tdrd3 Cell Signaling Cat# 5942S; RRID:AB_2797626 anti-vasa Developmental Studies Hybridoma Bank Cat# anti-vasa; RRID:AB_760351 anti-Hts 1B1 Developmental Studies Hybridoma Bank Cat# 1B1; RRID:AB_528070 anti-Top3b Lee et al. N/A anti-Tdrd3 Lee et al. N/A anti-Aub Nishida et al. N/A anti-Armi Cook et al. N/A anti-Yb Murota et al. N/A Chemicals, peptides, and recombinant proteins Shields and Sang M3 Insect Medium Sigma-Aldrich Cat# S3652-1L Fly Extract Drosophila Genomics Resource Center DGRC Stock 1645670 GlutaMAX gibco 35050061 Antibiotic-Antimycotic gibco 15240062 Glutathione Sigma-Aldrich G6013-25G Penicillin-Streptomycin gibco 15140122 Insulin Sigma-Aldrich I9278-5ml Paraformaldehyde Santa Cruz Biotechnology CAS 30525-89-4 Glutaraldehyde Sigma-Aldrich G7776-10ML X-Gal Solution Thermo Scientific R0941 TRIzol Invitrogen 15596026 SuperScript double-stranded cDNA synthesis kit Invitrogen 11917010 End-Repair Mix Qiagen Y9140-LC-L MinElute Reaction Cleanup kit Qiagen 28204 TBE-Urea Gels, 15% Invitrogen EC6885BOX Sodium Acetate Invitrogen AM9740 EDTA Invitrogen AM9260G Buffer EB Qiagen 19086 NEBNext Small RNA Library Prep kit New England Biolabs E7300 TaqMa MicroRNA Reverse Transcription Kit applied biosystems 4366597 Random Hexamers Invitrogen N8080127 SYBRTM Green Universal Master Mix applied biosystems 4309155 Triton X-100 Sigma-Aldrich X100-5ML HEPES gibco 15630080 MgCl2 invitrogen AM9530G NaCl invitrogen AM9760G Sucrose Sigma-Aldrich S0389-500G cOmplete Protease Inhibitor Cocktail Roche 11697498001 RNaseOU Recombinant Ribonuclease Inhibitor invitrogen 10777019 Anti-FLAG M2 Magnetic Beads Sigma-Aldrich M8823-1ML Chloroform Sigma-Aldrich C2432-25ML Critical commercial assays Mass spectrometry Taplin Biological Mass Spectrometry Facility https://taplin.hms.harvard.edu/ (Continued on next page) Cell Reports 44, 115495, April 22, 2025 19 ..
Magnetic Beads:Article Title: Topoisomerase 3b facilitates piRNA biogenesis to promote transposon silencing and germ cell development.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies a-mouse FLAG-M2 Sigma-Aldrich Cat# F3165; RRID:AB_259529 Mouse Tdrd3 Cell Signaling Cat# 5942S; RRID:AB_2797626 anti-vasa Developmental Studies Hybridoma Bank Cat# anti-vasa; RRID:AB_760351 anti-Hts 1B1 Developmental Studies Hybridoma Bank Cat# 1B1; RRID:AB_528070 anti-Top3b Lee et al. N/A anti-Tdrd3 Lee et al. N/A anti-Aub Nishida et al. N/A anti-Armi Cook et al. N/A anti-Yb Murota et al. N/A Chemicals, peptides, and recombinant proteins Shields and Sang M3 Insect Medium Sigma-Aldrich Cat# S3652-1L Fly Extract Drosophila Genomics Resource Center DGRC Stock 1645670 GlutaMAX gibco 35050061 Antibiotic-Antimycotic gibco 15240062 Glutathione Sigma-Aldrich G6013-25G Penicillin-Streptomycin gibco 15140122 Insulin Sigma-Aldrich I9278-5ml Paraformaldehyde Santa Cruz Biotechnology CAS 30525-89-4 Glutaraldehyde Sigma-Aldrich G7776-10ML X-Gal Solution Thermo Scientific R0941 TRIzol Invitrogen 15596026 SuperScript double-stranded cDNA synthesis kit Invitrogen 11917010 End-Repair Mix Qiagen Y9140-LC-L MinElute Reaction Cleanup kit Qiagen 28204 TBE-Urea Gels, 15% Invitrogen EC6885BOX Sodium Acetate Invitrogen AM9740 EDTA Invitrogen AM9260G Buffer EB Qiagen 19086 NEBNext Small RNA Library Prep kit New England Biolabs E7300 TaqMa MicroRNA Reverse Transcription Kit applied biosystems 4366597 Random Hexamers Invitrogen N8080127 SYBRTM Green Universal Master Mix applied biosystems 4309155 Triton X-100 Sigma-Aldrich X100-5ML HEPES gibco 15630080 MgCl2 invitrogen AM9530G NaCl invitrogen AM9760G Sucrose Sigma-Aldrich S0389-500G cOmplete Protease Inhibitor Cocktail Roche 11697498001 RNaseOU Recombinant Ribonuclease Inhibitor invitrogen 10777019 Anti-FLAG M2 Magnetic Beads Sigma-Aldrich M8823-1ML Chloroform Sigma-Aldrich C2432-25ML Critical commercial assays Mass spectrometry Taplin Biological Mass Spectrometry Facility https://taplin.hms.harvard.edu/ (Continued on next page) Cell Reports 44, 115495, April 22, 2025 19 ..
Mass Spectrometry:Article Title: Topoisomerase 3b facilitates piRNA biogenesis to promote transposon silencing and germ cell development.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies a-mouse FLAG-M2 Sigma-Aldrich Cat# F3165; RRID:AB_259529 Mouse Tdrd3 Cell Signaling Cat# 5942S; RRID:AB_2797626 anti-vasa Developmental Studies Hybridoma Bank Cat# anti-vasa; RRID:AB_760351 anti-Hts 1B1 Developmental Studies Hybridoma Bank Cat# 1B1; RRID:AB_528070 anti-Top3b Lee et al. N/A anti-Tdrd3 Lee et al. N/A anti-Aub Nishida et al. N/A anti-Armi Cook et al. N/A anti-Yb Murota et al. N/A Chemicals, peptides, and recombinant proteins Shields and Sang M3 Insect Medium Sigma-Aldrich Cat# S3652-1L Fly Extract Drosophila Genomics Resource Center DGRC Stock 1645670 GlutaMAX gibco 35050061 Antibiotic-Antimycotic gibco 15240062 Glutathione Sigma-Aldrich G6013-25G Penicillin-Streptomycin gibco 15140122 Insulin Sigma-Aldrich I9278-5ml Paraformaldehyde Santa Cruz Biotechnology CAS 30525-89-4 Glutaraldehyde Sigma-Aldrich G7776-10ML X-Gal Solution Thermo Scientific R0941 TRIzol Invitrogen 15596026 SuperScript double-stranded cDNA synthesis kit Invitrogen 11917010 End-Repair Mix Qiagen Y9140-LC-L MinElute Reaction Cleanup kit Qiagen 28204 TBE-Urea Gels, 15% Invitrogen EC6885BOX Sodium Acetate Invitrogen AM9740 EDTA Invitrogen AM9260G Buffer EB Qiagen 19086 NEBNext Small RNA Library Prep kit New England Biolabs E7300 TaqMa MicroRNA Reverse Transcription Kit applied biosystems 4366597 Random Hexamers Invitrogen N8080127 SYBRTM Green Universal Master Mix applied biosystems 4309155 Triton X-100 Sigma-Aldrich X100-5ML HEPES gibco 15630080 MgCl2 invitrogen AM9530G NaCl invitrogen AM9760G Sucrose Sigma-Aldrich S0389-500G cOmplete Protease Inhibitor Cocktail Roche 11697498001 RNaseOU Recombinant Ribonuclease Inhibitor invitrogen 10777019 Anti-FLAG M2 Magnetic Beads Sigma-Aldrich M8823-1ML Chloroform Sigma-Aldrich C2432-25ML Critical commercial assays Mass spectrometry Taplin Biological Mass Spectrometry Facility https://taplin.hms.harvard.edu/ (Continued on next page) Cell Reports 44, 115495, April 22, 2025 19 ..
Electron Microscopy:Article Title: Protocol to evaluate a lineage marking system in the Drosophila testis.
Article Snippet: The primary antibodies listed (anti-Vasa, 1b1, 7G10) are specific antibodies obtained from the DSHB and are critical for accurately identifying germline and niche cell populations. .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat monoclonal anti-Vasa (1:20) Developmental Studies Hybridoma Bank DSHB Cat# anti-vasa; RRID: AB_760351 Mouse monoclonal anti-hu-li tai shao (1:20) Developmental Studies Hybridoma Bank DSHB Cat# 1b1; RRID: AB_528070 Mouse monoclonal anti-Fasciculin III (1:40) Developmental Studies Hybridoma Bank DSHB Cat# 7G10 anti-Fasciclin III; RRID: AB_528238 Cyanine 3 goat anti-mouse IgG + IgM (H + L) (1:400) Jackson ImmunoResearch Labs RRID: AB_2338684 Cyanine 5 goat anti-rat IgG (H + L) (1:400) Jackson ImmunoResearch Labs RRID: AB_2338263 Chemicals, peptides, and recombinant proteins Formaldehyde, 16% Electron Microscopy Sciences Cat#15710 TritonX-100 Sigma Cat#X100-500ML 103 phosphate-buffered saline (PBS) Fisher Scientific Cat#BP3991 Bovine Serum Albumin (BSA) Sigma Cat#A2153-500G Poly-L-lysine Sigma Cat#P5899-5MG Schneider’s Drosophila medium Sigma Cat#21-720-024 Fetal Bovine Serum (FBS) Sigma Cat#NC3365964 Penicillin-Streptomycin-Glutamine (1003) Gibco (Fisher Scientific) Cat#10-378-016 VECTASHIELD with DAPI Vector Lab Cat#H-1200-10 Deposited data VCell math model Bener et al. 1 VCell model: Username: Boris; Model name: Inaba_dedifferentiation_model_public; https://vcell.org/run-vcell-software Experimental models: Organisms/strains D. melanogaster: nos-FRT-mCherrystop-FRT-Gal4, UAS-GFP (Reporter-1) Inaba lab stock 17 N/A D. melanogaster: UAS(FRT.stop)mCD8GFP (Reporter-2) Bloomington Drosophila Stock Center RRID: BDSC_ 30032 D. melanogaster: pVas-Vasa mCherry Gift from Yukiko Yamashita FBtp0065762 D. melanogaster: Bam-FLPD5 Bener et al. 1 RRID: BDSC_604597 Software and algorithms ZEN Microscopy Software ZEISS RRID:SCR_013672 ImageJ Fiji Schneider et al. 18 RRID:SCR_003070 https://imagej.net/software/fiji/ Virtual Cell Resasco et al. 19 ; Slepchenko and Loew. .. 20 RRID:SCR_007421 https://vcell.org/ Other Zeiss LSM800 confocal microscope with an Airyscan module Zeiss RRID:SCR_015963 35mm glass bottom dishes Nunc (Thermo Fisher) Cat#150680 Dissection microscope Leica M80 Fiber optic microscope illuminator Schott KL 1600 Dumont #55 forceps Fine Science Tools 1125520 Pyrex spot plate with nine depressions Corning Cat#7220-85 Drosophila flypads Genesee Scientific Cat#59-119 Drosophila CO 2 bubbler kits Genesee Scientific Cat#59-181 4 STAR Protocols 7, 104479, June 19, 2026 MATERIALS AND EQUIPMENT (A) An illustration of the Bam-FLPD5 lineage tracing system.
Saline:Article Title: Protocol to evaluate a lineage marking system in the Drosophila testis.
Article Snippet: The primary antibodies listed (anti-Vasa, 1b1, 7G10) are specific antibodies obtained from the DSHB and are critical for accurately identifying germline and niche cell populations. .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat monoclonal anti-Vasa (1:20) Developmental Studies Hybridoma Bank DSHB Cat# anti-vasa; RRID: AB_760351 Mouse monoclonal anti-hu-li tai shao (1:20) Developmental Studies Hybridoma Bank DSHB Cat# 1b1; RRID: AB_528070 Mouse monoclonal anti-Fasciculin III (1:40) Developmental Studies Hybridoma Bank DSHB Cat# 7G10 anti-Fasciclin III; RRID: AB_528238 Cyanine 3 goat anti-mouse IgG + IgM (H + L) (1:400) Jackson ImmunoResearch Labs RRID: AB_2338684 Cyanine 5 goat anti-rat IgG (H + L) (1:400) Jackson ImmunoResearch Labs RRID: AB_2338263 Chemicals, peptides, and recombinant proteins Formaldehyde, 16% Electron Microscopy Sciences Cat#15710 TritonX-100 Sigma Cat#X100-500ML 103 phosphate-buffered saline (PBS) Fisher Scientific Cat#BP3991 Bovine Serum Albumin (BSA) Sigma Cat#A2153-500G Poly-L-lysine Sigma Cat#P5899-5MG Schneider’s Drosophila medium Sigma Cat#21-720-024 Fetal Bovine Serum (FBS) Sigma Cat#NC3365964 Penicillin-Streptomycin-Glutamine (1003) Gibco (Fisher Scientific) Cat#10-378-016 VECTASHIELD with DAPI Vector Lab Cat#H-1200-10 Deposited data VCell math model Bener et al. 1 VCell model: Username: Boris; Model name: Inaba_dedifferentiation_model_public; https://vcell.org/run-vcell-software Experimental models: Organisms/strains D. melanogaster: nos-FRT-mCherrystop-FRT-Gal4, UAS-GFP (Reporter-1) Inaba lab stock 17 N/A D. melanogaster: UAS(FRT.stop)mCD8GFP (Reporter-2) Bloomington Drosophila Stock Center RRID: BDSC_ 30032 D. melanogaster: pVas-Vasa mCherry Gift from Yukiko Yamashita FBtp0065762 D. melanogaster: Bam-FLPD5 Bener et al. 1 RRID: BDSC_604597 Software and algorithms ZEN Microscopy Software ZEISS RRID:SCR_013672 ImageJ Fiji Schneider et al. 18 RRID:SCR_003070 https://imagej.net/software/fiji/ Virtual Cell Resasco et al. 19 ; Slepchenko and Loew. .. 20 RRID:SCR_007421 https://vcell.org/ Other Zeiss LSM800 confocal microscope with an Airyscan module Zeiss RRID:SCR_015963 35mm glass bottom dishes Nunc (Thermo Fisher) Cat#150680 Dissection microscope Leica M80 Fiber optic microscope illuminator Schott KL 1600 Dumont #55 forceps Fine Science Tools 1125520 Pyrex spot plate with nine depressions Corning Cat#7220-85 Drosophila flypads Genesee Scientific Cat#59-119 Drosophila CO 2 bubbler kits Genesee Scientific Cat#59-181 4 STAR Protocols 7, 104479, June 19, 2026 MATERIALS AND EQUIPMENT (A) An illustration of the Bam-FLPD5 lineage tracing system.
Software:Article Title: Protocol to evaluate a lineage marking system in the Drosophila testis.
Article Snippet: The primary antibodies listed (anti-Vasa, 1b1, 7G10) are specific antibodies obtained from the DSHB and are critical for accurately identifying germline and niche cell populations. .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat monoclonal anti-Vasa (1:20) Developmental Studies Hybridoma Bank DSHB Cat# anti-vasa; RRID: AB_760351 Mouse monoclonal anti-hu-li tai shao (1:20) Developmental Studies Hybridoma Bank DSHB Cat# 1b1; RRID: AB_528070 Mouse monoclonal anti-Fasciculin III (1:40) Developmental Studies Hybridoma Bank DSHB Cat# 7G10 anti-Fasciclin III; RRID: AB_528238 Cyanine 3 goat anti-mouse IgG + IgM (H + L) (1:400) Jackson ImmunoResearch Labs RRID: AB_2338684 Cyanine 5 goat anti-rat IgG (H + L) (1:400) Jackson ImmunoResearch Labs RRID: AB_2338263 Chemicals, peptides, and recombinant proteins Formaldehyde, 16% Electron Microscopy Sciences Cat#15710 TritonX-100 Sigma Cat#X100-500ML 103 phosphate-buffered saline (PBS) Fisher Scientific Cat#BP3991 Bovine Serum Albumin (BSA) Sigma Cat#A2153-500G Poly-L-lysine Sigma Cat#P5899-5MG Schneider’s Drosophila medium Sigma Cat#21-720-024 Fetal Bovine Serum (FBS) Sigma Cat#NC3365964 Penicillin-Streptomycin-Glutamine (1003) Gibco (Fisher Scientific) Cat#10-378-016 VECTASHIELD with DAPI Vector Lab Cat#H-1200-10 Deposited data VCell math model Bener et al. 1 VCell model: Username: Boris; Model name: Inaba_dedifferentiation_model_public; https://vcell.org/run-vcell-software Experimental models: Organisms/strains D. melanogaster: nos-FRT-mCherrystop-FRT-Gal4, UAS-GFP (Reporter-1) Inaba lab stock 17 N/A D. melanogaster: UAS(FRT.stop)mCD8GFP (Reporter-2) Bloomington Drosophila Stock Center RRID: BDSC_ 30032 D. melanogaster: pVas-Vasa mCherry Gift from Yukiko Yamashita FBtp0065762 D. melanogaster: Bam-FLPD5 Bener et al. 1 RRID: BDSC_604597 Software and algorithms ZEN Microscopy Software ZEISS RRID:SCR_013672 ImageJ Fiji Schneider et al. 18 RRID:SCR_003070 https://imagej.net/software/fiji/ Virtual Cell Resasco et al. 19 ; Slepchenko and Loew. .. 20 RRID:SCR_007421 https://vcell.org/ Other Zeiss LSM800 confocal microscope with an Airyscan module Zeiss RRID:SCR_015963 35mm glass bottom dishes Nunc (Thermo Fisher) Cat#150680 Dissection microscope Leica M80 Fiber optic microscope illuminator Schott KL 1600 Dumont #55 forceps Fine Science Tools 1125520 Pyrex spot plate with nine depressions Corning Cat#7220-85 Drosophila flypads Genesee Scientific Cat#59-119 Drosophila CO 2 bubbler kits Genesee Scientific Cat#59-181 4 STAR Protocols 7, 104479, June 19, 2026 MATERIALS AND EQUIPMENT (A) An illustration of the Bam-FLPD5 lineage tracing system.
Microscopy:Article Title: Protocol to evaluate a lineage marking system in the Drosophila testis.
Article Snippet: The primary antibodies listed (anti-Vasa, 1b1, 7G10) are specific antibodies obtained from the DSHB and are critical for accurately identifying germline and niche cell populations. .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat monoclonal anti-Vasa (1:20) Developmental Studies Hybridoma Bank DSHB Cat# anti-vasa; RRID: AB_760351 Mouse monoclonal anti-hu-li tai shao (1:20) Developmental Studies Hybridoma Bank DSHB Cat# 1b1; RRID: AB_528070 Mouse monoclonal anti-Fasciculin III (1:40) Developmental Studies Hybridoma Bank DSHB Cat# 7G10 anti-Fasciclin III; RRID: AB_528238 Cyanine 3 goat anti-mouse IgG + IgM (H + L) (1:400) Jackson ImmunoResearch Labs RRID: AB_2338684 Cyanine 5 goat anti-rat IgG (H + L) (1:400) Jackson ImmunoResearch Labs RRID: AB_2338263 Chemicals, peptides, and recombinant proteins Formaldehyde, 16% Electron Microscopy Sciences Cat#15710 TritonX-100 Sigma Cat#X100-500ML 103 phosphate-buffered saline (PBS) Fisher Scientific Cat#BP3991 Bovine Serum Albumin (BSA) Sigma Cat#A2153-500G Poly-L-lysine Sigma Cat#P5899-5MG Schneider’s Drosophila medium Sigma Cat#21-720-024 Fetal Bovine Serum (FBS) Sigma Cat#NC3365964 Penicillin-Streptomycin-Glutamine (1003) Gibco (Fisher Scientific) Cat#10-378-016 VECTASHIELD with DAPI Vector Lab Cat#H-1200-10 Deposited data VCell math model Bener et al. 1 VCell model: Username: Boris; Model name: Inaba_dedifferentiation_model_public; https://vcell.org/run-vcell-software Experimental models: Organisms/strains D. melanogaster: nos-FRT-mCherrystop-FRT-Gal4, UAS-GFP (Reporter-1) Inaba lab stock 17 N/A D. melanogaster: UAS(FRT.stop)mCD8GFP (Reporter-2) Bloomington Drosophila Stock Center RRID: BDSC_ 30032 D. melanogaster: pVas-Vasa mCherry Gift from Yukiko Yamashita FBtp0065762 D. melanogaster: Bam-FLPD5 Bener et al. 1 RRID: BDSC_604597 Software and algorithms ZEN Microscopy Software ZEISS RRID:SCR_013672 ImageJ Fiji Schneider et al. 18 RRID:SCR_003070 https://imagej.net/software/fiji/ Virtual Cell Resasco et al. 19 ; Slepchenko and Loew. .. 20 RRID:SCR_007421 https://vcell.org/ Other Zeiss LSM800 confocal microscope with an Airyscan module Zeiss RRID:SCR_015963 35mm glass bottom dishes Nunc (Thermo Fisher) Cat#150680 Dissection microscope Leica M80 Fiber optic microscope illuminator Schott KL 1600 Dumont #55 forceps Fine Science Tools 1125520 Pyrex spot plate with nine depressions Corning Cat#7220-85 Drosophila flypads Genesee Scientific Cat#59-119 Drosophila CO 2 bubbler kits Genesee Scientific Cat#59-181 4 STAR Protocols 7, 104479, June 19, 2026 MATERIALS AND EQUIPMENT (A) An illustration of the Bam-FLPD5 lineage tracing system.
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